Adventitious shoot regeneration in vitro from immature cotyledon of the sweet cherry.

The aim of this experiment was to examine the effects of the different development stage of cotyledon,6-BA and NAA concentration,cultivar and culture condition on adventitious shoot regeneration from the immature cotyledon explants of the sweet cherry(Prunus avium L.).The results showed that MS culture medium with 6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1 was the optimal for the adventitious shoot regeneration in the cultivar'Napoleon'and the optimal size of the immature cotyledon was PF(cotyledon length/embryo length×100)=50-80 and the regeneration rate reached to 79.2%.Dark culture for 2 weeks prior to light culture was helpful to adventitious shoot regeneration.Shoot regeneration rate also reached to 72.2% in 'Rainier',61.1% in 'Van'and 33.3% in 'Lapins'.Adventitious shoots were mainly regenerated at proximal cutting region of cotyledon adaxial surface.