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Comparison of Two Extraction Methods of Polysaccharides from HERBA EPHEDRAE

Qiuju Han

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Abstract

[Objective] To compare the extraction results of polysaccharide from HERBA EPHEDRAE by hot water extraction method and microwave-assisted method.[Method] The polysaccharide was extracted from HERBA EPHEDRAE respectively by hot water extraction method and microwave-assisted method,and their extraction conditions were optimized by orthogonal experiment.[Result] The best conditions of hot water extraction method were 60 ℃ temperature,3 h extraction duration and 1∶ 10(g/ml) solid-liquid ratio,under these conditions,the extraction rate of polysaccharide was 6.31%;the best conditions of microwave-assisted extraction method were 420 W microwave power,60 s extraction duration and 1∶ 10(g/ml) solid-liquid ratio,under these conditions,the extraction rate of polysaccharide was 5.57%.[Conclusion] The hot water extraction method yields high,and its conditions are easy to control,thus it is the best extraction method of polysaccharide from HERBA EPHEDRAE.

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What this paper is about

[Objective] To compare the extraction results of polysaccharide from HERBA EPHEDRAE by hot water extraction method and microwave-assisted method.[Method] The polysaccharide was extracted from HERBA EPHEDRAE respectively by hot water extraction method and microwave-assisted method,and their extraction conditions were optimized by orthogonal experiment.[Result] The best conditions of hot water extraction method were 60 ℃ temperature,3 h extraction duration and 1∶ 10(g/ml) solid-liquid ratio,under these conditions,the extraction rate of polysaccharide was 6.31%;the best conditions of microwave-assisted extraction method were 420 W microwave power,60 s extraction duration and 1∶ 10(g/ml) solid-liquid ratio,under these conditions,the extraction rate of polysaccharide was 5.57%.[Conclusion] The hot water extraction method yields high,and its conditions are easy to control,thus it is the best extraction method of polysaccharide from HERBA EPHEDRAE.

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Available abstract

[Objective] To compare the extraction results of polysaccharide from HERBA EPHEDRAE by hot water extraction method and microwave-assisted method.[Method] The polysaccharide was extracted from HERBA EPHEDRAE respectively by hot water extraction method and microwave-assisted method,and their extraction conditions were optimized by orthogonal experiment.[Result] The best conditions of hot water extraction method were 60 ℃ temperature,3 h extraction duration and 1∶ 10(g/ml) solid-liquid ratio,under these conditions,the extraction rate of polysaccharide was 6.31%;the best conditions of microwave-assisted extraction method were 420 W microwave power,60 s extraction duration and 1∶ 10(g/ml) solid-liquid ratio,under these conditions,the extraction rate of polysaccharide was 5.57%.[Conclusion] The hot water extraction method yields high,and its conditions are easy to control,thus it is the best extraction method of polysaccharide from HERBA EPHEDRAE.

Key concepts: Extraction (chemistry), Polysaccharide, Chromatography, Hot water extraction, Water extraction, Microwave, Microwave power, Chemistry

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