Extraction Process of Three Kinds of Chinese Herbs Polysaccharides

Objective:To study the difference of the process parameters with the three kinds of Chinese herbs polysaccharides by hot water extraction,and to investigate the possibility of combination extraction by orthogonal experiments.Method:Optimize extraction process of three kinds of polysaccharide respectively by orthogonal test,compare yield and water-solubility of polysaccharide extracted with optimal hot water extraction,alkali extraction and sonic extraction.Result:Optimal extraction processes of Rhizoma Chuanxiong polysaccharide was: the temperature of extraction was at 90 ℃,the ratio of sample to solution was 1∶20,and extraction time was 1 h.Optimal extraction processes of Radix Paeoniae Rubra polysaccharide was the temperature of extraction at 80 ℃,he ratio of sample to solution 1∶20,and extraction time 4 h.Optimal xtraction processes of Cortex Phellodendri Chinensis polysaccharide was extraction temperature 90 ℃,the ratio of sample to solution 1∶10,and extraction time 4 h.At the same time compared with polysaccharides extracted by the other two methods,polysaccharide extracted by hot water extraction has higher yield and solubility.Conclusion:The hot water extraction is more suitable for combination extraction.