Genetic Diversity of Chrysanthemum Cultivars Revealed by AFLP Analysis

The genetic diversity and relationship of 45 chrysanthemum cultivars were analyzed by AFLP (Amplified Fragment Length Polymorphism)-silver staining protocol. AFLP fingerprinting of 45 chrysanthemum cultivars with ten pairs of E+3/M+3 primers revealed a total number of 486 unambiguous bands, of which 451 were polymorphic and 45.1 polymorphic bands per pair of primer were produced on average. The polymorphism frequency was 92.80%. This result showed the abundant diversities of enzyme digestion sites among used chrysanthemum cultivars. As analyzed by DPSv3.01, the Nei's genetic distance of 45 chrysanthemum cultivars ranged from 0.36000 to 0.86237, and the average distance was 0.611185.These chrysanthemum cultivars were divided into six groups by UPGMA(unweighted pair group method with arithmetic average) based on Nei's genetic distances. The genetic relationship of 45 chrysanthemum cultivars was analyzed using the different coefficient.