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2007•Acta Horticulturae SinicaRequires access

Genetic Relationship of 85 Chrysanthemum〔Dendranthema×grandiflora(Ramat.) Kitamura〕Cultivars Revealed by ISSR Analysis

Miao HengBin, Fadi Chen, Hongbo Zhao

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Abstract

ISSR-PCR was used to detect the genetic diversity and relationship of 85 chrysanthemum cultivars. ISSR fingerprinting amplified by 22 ISSR primers revealed a total number of 160 unambiguous bands, of which 148 ones were polymorphic and the polymorphism frequency was 92.5%. As analyzed by POPGENE 1.31, the average value of effective number of alleles, Nei's gene diversity and Shannon's informa- tion index were 1. 6013,0. 3485 and 0. 5162, respectively. As analyzed by SPSS 11.0, the similarity coeffi- cient between cultivars ranged from 0. 294 to 0. 802. These 85 chrysanthemum cultivars were divided into six groups by UPGMA(unweighted pair group method with arithmetic average)based on Jaccard coefficient. The genetic relationship of 85 cultivars was related to their petal types.

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ISSR-PCR was used to detect the genetic diversity and relationship of 85 chrysanthemum cultivars. ISSR fingerprinting amplified by 22 ISSR primers revealed a total number of 160 unambiguous bands, of which 148 ones were polymorphic and the polymorphism frequency was 92.5%. As analyzed by POPGENE 1.31, the average value of effective number of alleles, Nei's gene diversity and Shannon's informa- tion index were 1. 6013,0. 3485 and 0. 5162, respectively. As analyzed by SPSS 11.0, the similarity coeffi- cient between cultivars ranged from 0. 294 to 0. 802. These 85 chrysanthemum cultivars were divided into six groups by UPGMA(unweighted pair group method with arithmetic average)based on Jaccard coefficient. The genetic relationship of 85 cultivars was related to their petal types.

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Available abstract

ISSR-PCR was used to detect the genetic diversity and relationship of 85 chrysanthemum cultivars. ISSR fingerprinting amplified by 22 ISSR primers revealed a total number of 160 unambiguous bands, of which 148 ones were polymorphic and the polymorphism frequency was 92.5%. As analyzed by POPGENE 1.31, the average value of effective number of alleles, Nei's gene diversity and Shannon's informa- tion index were 1. 6013,0. 3485 and 0. 5162, respectively. As analyzed by SPSS 11.0, the similarity coeffi- cient between cultivars ranged from 0. 294 to 0. 802. These 85 chrysanthemum cultivars were divided into six groups by UPGMA(unweighted pair group method with arithmetic average)based on Jaccard coefficient. The genetic relationship of 85 cultivars was related to their petal types.

Key concepts: UPGMA, Cultivar, Petal, Genetic diversity, Jaccard index, Biology, Genetic relationship, Genetic similarity

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Genetic Relationship of 85 Chrysanthemum〔Dendranthema×grandiflora(Ramat.) Kitamura〕Cultivars Revealed by ISSR Analysis — Research Paper | ScholarLens