Optimization of Extraction and Purification Technology for Total Flavones from Aster ageratoides

Objective:To optimize extraction and purification technology of total flavones from Aster ageratoides.Method:With the content of total flavones as index,extraction technology conditions of total flavones was optimized by orthogonal test;Type of macroporous resin was selected with static adsorption capacity and desorption rate as indexes,and to optimized purification technology of total flavones.Result:Optimum extraction conditions were as follows:soaking extracted 3 times with 10 times the amount of 60% ethanol at 50 ℃.HPD-500 type macro porous resin showed better adsorption and desorption property,optimum purification conditions were as follows:sample solution was prepared with the concentration of 50 g · L-1,subjected to HPD-500 type macroporous resin column chromatography with load ratio of 16 mg total flavones per gram of resin,standing for 1 hour,column was eluted with 4 BV water before being eluted with 2 BV 70% ethanol.Under these conditions,purity of total flavones was 76.7%,which enhanced 3.83 times.Conclusion:This optimized technology was convenient and practical,it could provide a reference for industrial production of A.ageratoides.