Changes and Role of Endogenous Hydrogen Sulfide in Rat with Heart Failure

Objective To investigate the changes of endogenous hydrogen sulfide(H2S)in rat with heart failure(HF)and the effects of exogenous administration of H2S donor sodium hydrosulphide(NaHS)on myocardial infarction,so as to explore the pathophysiological significance of H2S in HF.Methods: The experimental HF model was induced by left anterior descending coronary occlusion and then was treated with intraperitoneal injection of H2S;the degree of myocardial infarction was determined by observing hematoxylin-eosin stained slices;the H2S level was measured by methylene blue;the contents of malondialdehyde(MDA)in plasma and myocardial homogenates were detected by TBA;aspartate amino transferase(AST),creatine phosphokinase(CPK)and lactate dehydrogenase(LDH)activities in serum were determined by automatic biochemistrical analysis apparatus.Results: Severe myocardial infarction was induced by coronary occlusion;H2S production in plasma and heart homogenates were significantly declined(P0.001);the content of MDA in plasma and heart homogenates was increased(P0.05);AST,CPK and LDH activities in plasma were increased(each P0.05).After treatment with NaHS,myocardial infarction was obviously reduced;the contents of MDA in plasma and heart homogenates were markedly declined(each P0.05);AST,CPK and LDH activities in plasma were decreased(each P0.05).Conclusion: The results suggest that H2S has protective effects in HF following myocardial ischemia in rats by decreasing MDA production in plasma and heart.