Protection of exogenous hydrogen sulfide against isolated rat heart injury perfused with hydrogen peroxide

OBJECTIVE To investigate the protective effect of exogenous hydrogen sulfide on the hydrogen peroxide( H 2 O 2)-induced injury in isolated rat hearts. METHODS After a 20 min perfusion with K-H solution in Langendorff perfusion system,hearts in model group were subjected to K-H solution containing H 2 O 2 200 μmo·lL- 1for 10 min,hearts in H 2 O 2 + NaHS group,received 10 min of H 2 O 2 200 μmo·lL- 1 containing buffer followed by another 10 min of NaHS 10 μmol·L- 1. Treated with different materials,all hearts were exposed to 60 min repufusion with K-H solution. The heart rate( HR),left ventricular systolic pressure( LVSP) and left ventricular end diastolic pressure( LVEDP) were recorded. The activity of lactate dehydrogenase( LDH),malondialdehyde( MDA) and superoxide dismutase( SOD) in coronary effluent fluid was determined. After reperfusion,the rat hearts were removed for HE staining to detect the histomorphological changes in myocardial tissues. RESULTS No differences in baseline hemodynamics were observed among the experimental groups. Compared with model group,H 2 O 2 + NaHS group showed significantly better recovery of hemodynamic function. These improvements were paralleled with the reduction of the activity of LDH〔( 884 ± 51) vs( 1290 ± 71) U·L- 1〕and MDA〔( 7.58 ±0.59) vs( 9. 3 ± 0. 6) nmo·lL- 1〕in the coronary effluent fluid,and the activity of SOD increased〔( 90 ±11) vs( 69 ± 8) U·L- 1〕( P 0.05),the myocardial damage was attenuated. CONCLUSION Exogenous hydrogen sulfide has protective effect on the H 2 O 2-induced injury in isolated rat hearts.