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2010•Biotechnology(Faisalabad)Requires access

Cloning and Characterization of Ubiquitin Extension Protein Gene in Paeonia suffruticosa

Dong Li

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Abstract

Objective:Cloning of ubiquitin extension gene from Paeonia suffruticosa,was the foundation of further research and gene expression study.Method:Total RNA was extracted from leaves,petals and sepals.Then reverse transcripted by M-MLV.Result:A 389bp fragment was obtained by Nest PCR,which encoded 129 amino acid residues.Conclusion:The fragment showed 100% identities with Arabidopsis thaliana,Solanum tuberosum,Oryza sativa,et al,while similarities of the deduced amino acid were more than 94% .

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Objective:Cloning of ubiquitin extension gene from Paeonia suffruticosa,was the foundation of further research and gene expression study.Method:Total RNA was extracted from leaves,petals and sepals.Then reverse transcripted by M-MLV.Result:A 389bp fragment was obtained by Nest PCR,which encoded 129 amino acid residues.Conclusion:The fragment showed 100% identities with Arabidopsis thaliana,Solanum tuberosum,Oryza sativa,et al,while similarities of the deduced amino acid were more than 94% .

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Available abstract

Objective:Cloning of ubiquitin extension gene from Paeonia suffruticosa,was the foundation of further research and gene expression study.Method:Total RNA was extracted from leaves,petals and sepals.Then reverse transcripted by M-MLV.Result:A 389bp fragment was obtained by Nest PCR,which encoded 129 amino acid residues.Conclusion:The fragment showed 100% identities with Arabidopsis thaliana,Solanum tuberosum,Oryza sativa,et al,while similarities of the deduced amino acid were more than 94% .

Key concepts: Paeonia suffruticosa, Cloning (programming), Gene, Biology, Arabidopsis thaliana, Petal, Oryza sativa, Molecular cloning

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