Cloning and Characterization of Ubiquitin Extension Protein Gene Fragment of Mulberry

Objective:Cloning plant ubiquitin extension protein gene with 3' RACE,that was the foundation of researching function.Methods:Total RNA was extracted from the young leaves of Morus bombycis and transcribed into cDNA.Using one universal primer and another designed from reported ubiquitin gene sequences,a 690 bp cDNA fragment were amplified with 3' RACE(Rapid Amplification of cDNA End) method.Results:The fragment contains a 3' UTR(Untranslated Region) 219 bp in length and a 5' ORF(Open Reading Frame) which encodes 156 deduced amino acid residues.Conclusion:The deduced amino acid sequence showed more than 96% similarity with the corresponding ubiquitin protein fragment of Solanum tuberosum,Nitotiana tabacum,Gossypium hirsutum,C.sativus and ribosomal S27A protein of M.multicaulis.The sequence information of the fragment indicates that this is the ubiquitin gene fragment.