Cloning and expression analysis of the ubiquitin extension protein gene fragment of Paeonia suffruticosa

A cDNA fragment of ubiquitin extension protein gene was obtained from petals of Paeonia suffruticosa by RT-PCR,which was named PsUBQ and registered in GenBank with accession no.JN699053.This fragment was 423 bp in length and encoded 140 amino acids,which contained a ubiquitin monomer fused with a ribosomal S27a protein.Multiple sequence alignment indicated that the PsUBQ protein was highly similar to the homologous proteins of other higher plant species such as Arabidopsis thaliana,Prunus avium and Vitis vinifera.Real-time PCR analysis showed that PsUBQ was presented in different tissues of Paeonia suffruticosa with stable expression,and constitutively expressed in petals during cut flower opening.The cloning and expression analysis of Paeonia suffruticosa ubiquitin extension protein gene provides a basis for further studying the function of ubiquitin in hormone signaling during flower opening and senescence,and also serves as a reference for normalization of target gene expression in Paeonia suffruticosa.