Role of Leptin on the expression of Survivin in breast cancer cells

OBJECTIVE:To detect the effect of lepin on Survivin expression in breast cancer cells(T47D).METHODS:To collect breast cancer cells T47D in the logarithmic growth phase,and culture them at 5×104 / well in 48-well plates,respectively,in the following processing.C group:the control group,without any treatment after starvation;L group:cells treated with 10 nmol/L Leptin.The Survivin protein expression was quantified by Western-blot assay.In addition,the Survivin mRNA expression was detected by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR).RESULTS:Western blot analysis showed that Survivin protein expression in T47D cells was upregulated afer treatment by 10 nmol/L Leptin for 2 h.RT-PCR showed that Leptin(10 nmol/L) could promote the expression of Survivin mRNA in T47D cells after treatment for 30 min and 60 min,compared with the control group.After treatment by Leptin(10 nMol / L) for 30 min and 60 min,Survivin mRNA expression was 2.15±0.19 and 4.55±0.23 times higher than those of the control group.CONCLUSION:Leptin increases the expression of Survivin in T47D breast cancer cells.