Influence of artesunate on expression of survivin in breast cancer MDA-MB-231 cells

Objective To study the influence of artesunate on the expression of survivin in breast cancer MDA-MB-231 cells.Methods The different concentrations(0,25,50 μg/mL) of artesunate were used to treat the MDA-MB-231 cells.The cells and supernatant were collected at 24,48,72 h after treating by different concentrations of drugs.RT-PCR was used to detect the mRNA level of survivin,and the secreted protein level of survivin was determined by ELISA kit.Results Both the results of PCR and ELISA displayed that with the increasing of drug′s concentration or the extending of treating time,the gene level and protein level of survivin analyzed by one-way ANOVA were gradually reduced,showing statistical difference among experimental groups in the same time(P0.05).There was no difference of survivin′s expression at 24 h and 48 h in control group(0 μg/mL) analyzed by t-test.But the expression of survivin in each drug treated group was decreased more conspicuously at 48 h than at 24 h,t-test showing statistical difference(P0.05).Conclusion Artesunate can induce the MDA-MB-231 cells to apoptosis through inhibiting the expression of survivin.