Effects of hepatocyte growth factor on foot cell nephrin expression in adriamycin nephrosis rats

Objective To observe the effect of hepatocyte growth factor(HGF)on foot cell nephrin expression in adriamycin nephrosis rats.Methods 114male SD rats of clean grade were divided into the control group(n=42),model group(n=42)and intervention group(n=30),control group injected intravenously through tail vein once an equal volume of saline solution;the experimental group and the intervention group injected intravenously through tail vein with adriamycin solution to establish a one-time cyclosporine nephropathy model,the intervention group for third weeks after modeling was given by intraperitoneal injection of HGF until the end of the experiment;the rats of control group and the model group sacrificed after first,2,3,4,6,8and 12weeks after successful animal modeling.The rats of intervention group sacrificed in the injection of HGF after the first week,namely after 3weeks,4weeks,6weeks,8weeks and 12weeks after successful animal modeling,the day before sacrifice,24hour urinary protein,renal tissue were detected by immunofluorescence staining,the expression intensity of nephrin was tested by using fluorescence confocal microscopy detection of the glomeruli in podocytes.Results Compared with the control group,urine protein in model group increased significantly,urine protein after first,2,3,4,6,8and 12 weeks after successful animal modeling,respectively(854.3±77.0)mg/L vs(330.6±33.1)mg/L,(814.8±158.6)mg/L vs(343.4±77.0)mg/L,(795.0±242.3)mg/L vs(415.6±113.5)mg/L,(703.5±137.3)mg/L vs(355.1±84.8)mg/L,(621.7±241.2)mg/L vs(350.4±61.8)mg/L,(751.4±389.9)mg/L vs(369.3±62.7)mg/L,(725.4±170.2)mg/L vs(435.2±140.9)mg/L,respectively(P 0.01),given the intervention of HGF,urinary protein significantly reduced(P 0.01).Compared with the control group,nephrin model group exposed more increase significantly(P 0.01);HGF intervention after nephrin exposure significantly reduced compared with the model group(P 0.01).Conclusion Urinary protein in adriamycin induced nephropathy rats increases significantly,nephrin exposure shows first increase and then decrease;HGF can reduce acute stage podocyte injury,exert protective effect on podocytes,alleviate the pathological damage of kidney by adriamycin nephropathy in rats,decrease the urine protein.