Study on Apoptosis of Human Cervical Carcinoma Hela Cell Induced by Bufonin and Mitomycin

Objective To approach the apoptosis mechanism of the anti-cancer drugs of bufonin and mitomycin to human cervical carcinoma cell Hela.Methods Human cervical carcinoma Hela cells were cultured in vitro,and stimulated with different concentration of bufonin and mitomycin.The cell morphology was observed under the inverted microscope,the cell proliferation was detected by MTT.DNA gragmentation was detected by agarose gel electrophoresis.Cell apoptosis was inspected by FITC-Annexin V.In addition,the expression of TNF-α in Hela cell was observed by RT-PCR.Results After treating by the drugs for six hours,the cell apoptosis was apparently appeared,the result of the apoptotic DNA was presented to a 200bp fragment after the agarose gel electrophoresis.In the prophase of the apoptosis,the membrane was dyed into green fluorescence,after 12 hours,the nucleus was dyed by propidium iodide gradually,and after 24 hours,most of the cells were dyed into red color.The product of the RT-PCR was 299bp.Conclusions The combined use of bufonin and mitomycin can inhibit the cell growth,induce the apoptosis of the Hela cell,and induce fragments of DNA and expression of TNF-α.