Determination of Oleanolic Acid and Ursolic Acid in Houyanning Tablets by HPLC

Objective To establish an HPLC method for the determination of oleanolic acid and ursolic acid in Houyanning tablets.Methods HPLC was performed on Diamonsil C18(250 mm×4.6 mm,5 μm) at room temperature,the mobile phase was methanol-water-acetic acid(80∶20∶0.5).The flowing rate was 1.0 ml/min,and the detection wavelength was 215 nm,the amount of input was 20 μl.Results The content of oleanolic acid and ursolic acid showed a good linearity in the range of 7.31～73.1 μg(r=0.999 8) and 24.27～242.7 μg(r=0.999 7),the average recoveries of oleanolic acid and ursolic acid were 99.61%(RSD=1.06%) and 99.82(RSD=1.21%).Conclusion This method is simple and accurate with good recurrence,and can be used for the quality control of the Houshuning tablets.