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Content compare of ursolic acid and oleanolic acid in different spot of Perillalinn

Chen Wu

Abstract

Ursolic acid and oleanolic acid from Perillalinn were extracted by supersonic technology,and RP-HPLC method for the determination of ursolic acid and oleanolic acid was developed.Ursolic acid and oleanolic acid was separated and analyzed on Kromasil C_ 18 column(250mm×4.6mm,5μm) using methanol-water(87∶13) as mobile phase at flow rate of 0.8mL/min.UV detection was at 210nm and the column temperature was 28℃.An average recovery of 98.53% was obtained with a RSD of 1.3% in the range of 0.359～5.744μg for ursolic acid,and an average recovery of 98.36% was obtained with a RSD of 1.5% in the range of 0.174～2.784μg for oleanolic acid.HPLC method was proved to be sensitive,fast and accurate It can be used for the determination of ursolic acid and oleanolic acid in Perillalinn.

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Key concepts: Ursolic acid, Oleanolic acid, Chromatography, Chemistry, High-performance liquid chromatography, Methanol, Organic chemistry, Medicine