2006•Zhongliu fangzhi yanjiuRequires access
The Influence of Arsenic Trioxide on the Growth and Telomerase Activity of Human Large Intestine Carcinoma Cells
Lin Liu
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Abstract
Objective To study the influence of arsenic trioxide on the growth and telomerase activity of human large intestine carcinoma cell lines. Methods The influence of growth on human large intestine carcinoma cell lines LoVo and CoLo-320 induced by As_ 2 O_ 3 injection was analyzed by observing living cells method, Trypan blue method, 3-(4,5- dimethylthiazole-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Telomerase activity of the carcinoma cells was measured by the method of repeat amplitication protocol (TRAP) combining with polyacrylamide gel electrophosis (PAGE) silver staining method before and after the treatment with As_ 2 O_ 3 injection. Results As_ 2 O_ 3 injection could strongly inhibit the growth of human large intestine carcinoma cell lines LoVo and CoLo-320.Also the tolemerase activity of LoVo and CoLo-320 treated by As_ 2 O_ 3 injection was inhibited obviously, which showed both dose-dependent and time-dependent relationship. Conclusion As_ 2 O_ 3 has significant inhibition effect on the human large intestine carcinoma cell lines, which maybe closely related to As_ 2 O_ 3 's_ inhibiting effect on the carcinoma cell tolemerase activity.