Construction and Detection of Retrovirus Vector with miR367 in Mouse

The aim of this study was to construct the retroviral vector with mouse miR367 and construction its mechanism of action in cell reprogramming.miR367 was cloned from mouse genome,and then linked with vector pMD18-T.The recombinant plasmid was digested and the segment with miR367 was purified,then subcloned into retroviral vector pMXs.Plat-E cells were transfected by the vector.Viral suspension was infected MEF cells in order to detect the expression of miR367.PCR and restriction enzyme digestion revealed that miR367-pMXs plasmid was constructed successfully.Q-PCR revealed that the expression of miR367 was enhanced in the infected-MEF.Result showed that mouse miR367 retroviral vector had been successfully constructed,and it had laid a good foundation for further research of cell reprogramming.