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2010•Neural Injury and Functional ReconstructionRequires access

Construction and Identification of a Retroviral Vector Expressing MicroRNA302a

Sumin Zhang

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Abstract

Objective:To construct a retroviral vector expressing miR-302a to investigate the role of micro-RNA in cellular reprogramming.Methods:miR-302a precursor sequence was amplified from the mice genomic DNA,inserted into pMX-IRES-GFP vector and identified by DNA sequencing.PLAT-E cells were transfected with the pMx-miR302a-IRES-GFP and the titer of virus was tested by the expression of GFP.Results:It was demonstrated by DNA sequencing that the retroviral vector pMx-miR302a-IRES-GFP was successfully constructed.The titer of concentrated virus was(1~5)×106 TU/mL.Conclusion:The retroviral vector pMx-miR302a-IRES-GFP was successfully constructed.

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What this paper is about

Objective:To construct a retroviral vector expressing miR-302a to investigate the role of micro-RNA in cellular reprogramming.Methods:miR-302a precursor sequence was amplified from the mice genomic DNA,inserted into pMX-IRES-GFP vector and identified by DNA sequencing.PLAT-E cells were transfected with the pMx-miR302a-IRES-GFP and the titer of virus was tested by the expression of GFP.Results:It was demonstrated by DNA sequencing that the retroviral vector pMx-miR302a-IRES-GFP was successfully constructed.The titer of concentrated virus was(1~5)×106 TU/mL.Conclusion:The retroviral vector pMx-miR302a-IRES-GFP was successfully constructed.

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Available abstract

Objective:To construct a retroviral vector expressing miR-302a to investigate the role of micro-RNA in cellular reprogramming.Methods:miR-302a precursor sequence was amplified from the mice genomic DNA,inserted into pMX-IRES-GFP vector and identified by DNA sequencing.PLAT-E cells were transfected with the pMx-miR302a-IRES-GFP and the titer of virus was tested by the expression of GFP.Results:It was demonstrated by DNA sequencing that the retroviral vector pMx-miR302a-IRES-GFP was successfully constructed.The titer of concentrated virus was(1~5)×106 TU/mL.Conclusion:The retroviral vector pMx-miR302a-IRES-GFP was successfully constructed.

Key concepts: Internal ribosome entry site, Viral vector, Transfection, Titer, Biology, Green fluorescent protein, Vector (molecular biology), Virology

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