Construction and detection of retrovirus vector with mouse miR-16

The aim of this study was to construct the eukaryotic expression vector with mouse microRNA16 and construction its mechanism of action in cell reprogramming. miRNA- 16 was amplified from mouse genome,and then linked with vector p MD18- simple. The recombinant plasmid was digested and the segment with miR- 16 was purified then subcloned into retroviral vector p MXs. Plat- E cells were transfected by the vector. Viral suspension was infected MEF cells in order to detect the expression of miR- 16. Result PCR and restriction enzyme digestion revealed that miR16- p MXs plasmid was constructed successfully. q PCR revealed the expression of miR- 16 was significantly enhanced in the infected- MEF.Conclusion: Mouse miR- 16 retroviral vector has been successfully constructed,and it has laid a good foundation for further research of cell reprogramming.