Influence of nuclear factor-κB inhibitor PDTC on polymorphonuclear neutrophil apoptosis in rats with acute lung injury

OBJECTIVE To explore the influence of nuclear factor-KB inhIbitor PDTC on the polymorphonuclear neutrophil(PMN) apoptosis in rats with acute lung injury(ALI) induced by lipopolysaccharide(LPS).METHODS A total of 54 male rats were randomly divided into 3 groups.The rats in the control group N(n=6) were injected into the peritoneal cavity with 3 ml/kg of normal saline,ALI group(group L,n=24) were injected into peritoneal cavity with 3mg/kg of LPS;PDTC group(P plus L group,n=24) were firstly injected into peritoneal cavity with 120 mg/kg of PDTC,and then 3mg/kg of LPS at 30min after injecting with PDTC,the samples of the two groups were observed for the blood gas analysis,determination of lung water content(W/D),and PMN apoptosis rate in bronchoalveolar lavage fluid(BALF) at 2h,4h,8h,and 12h after injecting with LPS.RESULTS As compared with group N,PaO2 decreased significantly in group L and was better in group P plus L(P0.05).W/D increased more significantly in L and P plus L groups than group N,significantly decreasing in P plus L group as compared with group L;the apoptotic rate of PMN in BALF decreased more significantly in group L and group P plus L than group N,as compared with the apoptotic rate of PMN in BALF of group P plus L group and group L at corresponding time points,the difference was statistically significant(P0.05).CONCLUSION PDTC can effectively relieve the ALI induced by LPS through promoting the apoptosis of PMN.