Influence of pyrrolidine dithiocarbamate on the tumor necrosis factor-α in rats with acute lung injury

Objective To investigate the influence of pyrrolidine dithiocarbamate(PDTC) on the tumor necrosis factor-α in rats with acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods A total of 66 male rats were randomly divided into 3 groups.The rats in control group(N group,n=6) were injected into the peritoneal cavity with normal saline,in lung injury group(L group,n=30) with LPS to reproduce ALI model,and in PDTC group(P+L group,n=30) with LPS and PDTC.The samples of L group and P+L group were taken for TNF-α in lung homogenate and blood plasma at 1 h,2 h,4 h,8 h and 12 h.Result Compared with N group,TNF-α increased significantly in L group(P0.01) and was better in P+L group(P0.05).Conclusions LPS caused amount of TNF-α release in lung homogenate and blood.It suggestes that NF-κB involves in control of inflammation and plays an important role in ALI.PDTC can control the expression and release of inflammation,and effectively relieve the ALI induced by LPS.