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2011•Journal of Wuhan Institute of TechnologyRequires access

Determination of cycloxylidin by HPLC

JU Xiu-lian

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Abstract

The method for the determination of Cycloxylidin by high performance liquid chromatography(HPLC) was established.It used C18 column(250 mm×4.6 mm,5 μg/mL) to separate the sample,25% methanol and 75% water as the mobile phase,and the Cycloxylidin was detected at 220 nm with the flow rate of 1.0 mL/min at ambient temperature.The result showed that the linear relative coefficient was 0.999 92 while in a range of 43.2—345.6 μg/mL;the limit of this method was 0.4 μg.This method was simple,rapid and effective in the quality control for production of Cycloxylidin.

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What this paper is about

The method for the determination of Cycloxylidin by high performance liquid chromatography(HPLC) was established.It used C18 column(250 mm×4.6 mm,5 μg/mL) to separate the sample,25% methanol and 75% water as the mobile phase,and the Cycloxylidin was detected at 220 nm with the flow rate of 1.0 mL/min at ambient temperature.The result showed that the linear relative coefficient was 0.999 92 while in a range of 43.2—345.6 μg/mL;the limit of this method was 0.4 μg.This method was simple,rapid and effective in the quality control for production of Cycloxylidin.

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Available abstract

The method for the determination of Cycloxylidin by high performance liquid chromatography(HPLC) was established.It used C18 column(250 mm×4.6 mm,5 μg/mL) to separate the sample,25% methanol and 75% water as the mobile phase,and the Cycloxylidin was detected at 220 nm with the flow rate of 1.0 mL/min at ambient temperature.The result showed that the linear relative coefficient was 0.999 92 while in a range of 43.2—345.6 μg/mL;the limit of this method was 0.4 μg.This method was simple,rapid and effective in the quality control for production of Cycloxylidin.

Key concepts: Chromatography, High-performance liquid chromatography, Linear range, Methanol, Analytical Chemistry (journal), Chemistry, Detection limit, Volumetric flow rate

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