A Stability Indicating HPLC Method for the Determination of Amodiquine Hydrochloride

Stability indicating HPLC method has been developed for specific determination of Amodiquine hydrochloride during degradation under acid, alkali, thermal and oxidative stress conditions. Acn: Phosphate buffer pH (5.8) with ortho phosphoric acid (80:20), was used as the mobile phase, at a flow rate of 1 ml/min. A Grace smart C18 column (5µ), 344 mm, 2.6 mm i.d. was used as stationary phase. The retention time was approximately 2.6 min. Linearity range was 10-50 µg/ml. The minimum detection and Quantitation limits were 0.1605 and 0.53 µg/ml respectively. Proposed method was validated for precision, accuracy, ruggedness, robustness and specificity.