Epidemiological research of infantile diarrhea with rotavirus in Hohhot,2008-2009

Objective To study the epidemiological characteristics of infantile diarrhea with rotavirus in Hohhot from June 2008 to May 2009. Methods A total of 313 stool specimens were collected from children under 5 years old with acute diarrhea who were hospitalized in Inner Mongolia Maternal and Child Health Hospital from June 2008 to May 2009.Enzyme linked immunosorbent assay(ELISA) was used to detect rotavirus genemic RNA.Nested reverse transcription polymerase chain reaction(nested RT-PCR) was performed to identify rotavirus VP7(G1、G2、G3、G4、G8、G9) and VP4(P[8]、P[4]、P[6]、P[9]、P) genotypes.SPSS 13.0 were applied for data analysis.Results 313 stool specimens,125 were detected rotavirus positive, the infection rate was 39.94%.Among 125 positive samples G1(64.8%) was the most predominants train,followed by G1+G3(16.8%),G3(14.4%),G2(2.4%),G9(0.8%) and G1+G2(0.8%);P genotyping showed P[8](84.0%) was the most common,followed by P[4](4.0%),P[8]+P[4](1.6%),13 strains were nontypeable.Conclusions Rotavirus were one of the most common cause of acute gastroenteritis among infants in Huhhot.G1P[8] was the main prevalent serotype.For the first time in Inner Mongolia region,G9 serotype was detected.