Determination of total flavonoids in three natural products
Han Xiu-ping
Abstract
Han Xiu-ping
Abstract
The flavonoids in three natural products (including okra leaves, rice leaves, Theorny amaranth) were determined by UV-visible spectrophotometry. The flavonoids in three natural products were ultrasonic extracted with 70% ethanol. The standard curve A=0.01175C-0.02247 of Rutin was extablished by spectrophotometry at 505 nm when the color reagent was admixtures of NaNO2 -Al(NO 3 ) 3 -NaOH solution. Data between the flavonoids content and absorbance had a better linear relationship r=0.9998. The total flavonoids in three natural products are okra leaves 34.40 mg/g, rice leaves20.26 mg/g, Theorny amaranth2.34 mg/g.
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The flavonoids in three natural products (including okra leaves, rice leaves, Theorny amaranth) were determined by UV-visible spectrophotometry. The flavonoids in three natural products were ultrasonic extracted with 70% ethanol. The standard curve A=0.01175C-0.02247 of Rutin was extablished by spectrophotometry at 505 nm when the color reagent was admixtures of NaNO2 -Al(NO 3 ) 3 -NaOH solution. Data between the flavonoids content and absorbance had a better linear relationship r=0.9998. The total flavonoids in three natural products are okra leaves 34.40 mg/g, rice leaves20.26 mg/g, Theorny amaranth2.34 mg/g.
Key concepts: Rutin, Spectrophotometry, Chemistry, Amaranth, Absorbance, Flavonoid, Reagent, Chromatography