HMW-GS Composition in the Representative Wheat Landraces from China
Xiuquan Li
Abstract
Xiuquan Li
Abstract
The high molecular weight glutenin(HMW-GS) composition of 200 representative wheat(Triticum aestivum L.) landraces originated from 22 provinces and regions of china were analyzed by sodium dodecyl sulphate polyacrylamide-gel electrophoresis.Thirty-five kinds of glutenin patterns were observed.null,7+8,2+12 was the most frequent subunit combination(59%).The frequencies of the other subunit combinations are all less than 10%.The number of alleles on Glu-A1,Glu-B1,Glu-D1 loci are 3,12 and 9.The predominant subunits at the three loci are null,7+8,2+12 and their frequencies are 86.5%,73.0% and 85.0%,respectively.The results indicated that there were various HMW-GS subunit combinations in Chinese wheat landraces,however,the frequencies of the high quality subunits were low.In addition,some rare subunit combinations such as6+8+19 and 6+9,7,22 were detected in this study.The number of landraces with quality scores above 8 among 200 wheat landraces is 22.The number of landraces with quality scores above 9 among 200 wheat landraces is 4,they are Caoxiebian(ZM011396)、Zhong81Ⅲ-6218(ZM013120)、Gao8(ZM009428)and Jinghong 7 hao(ZM008935).
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The high molecular weight glutenin(HMW-GS) composition of 200 representative wheat(Triticum aestivum L.) landraces originated from 22 provinces and regions of china were analyzed by sodium dodecyl sulphate polyacrylamide-gel electrophoresis.Thirty-five kinds of glutenin patterns were observed.null,7+8,2+12 was the most frequent subunit combination(59%).The frequencies of the other subunit combinations are all less than 10%.The number of alleles on Glu-A1,Glu-B1,Glu-D1 loci are 3,12 and 9.The predominant subunits at the three loci are null,7+8,2+12 and their frequencies are 86.5%,73.0% and 85.0%,respectively.The results indicated that there were various HMW-GS subunit combinations in Chinese wheat landraces,however,the frequencies of the high quality subunits were low.In addition,some rare subunit combinations such as6+8+19 and 6+9,7,22 were detected in this study.The number of landraces with quality scores above 8 among 200 wheat landraces is 22.The number of landraces with quality scores above 9 among 200 wheat landraces is 4,they are Caoxiebian(ZM011396)、Zhong81Ⅲ-6218(ZM013120)、Gao8(ZM009428)and Jinghong 7 hao(ZM008935).
Key concepts: Glutenin, Biology, Common wheat, Protein subunit, Polyacrylamide gel electrophoresis, Allele, Composition (language), Agronomy