Study on extracting process of Pueraria flavone and evaluation of anti-oxidant activity

The optimal process of extracting flavone from Pueraria by ethanol was studied in this paper,and the total content of flavones was 11.7%.Meanwhile,the anti-oxidant activities of the extraction liquid were also studied by Luminol-H2O2 chemiluminescence system and the optimal conditions were obtained by orthogonalmatrix on the basis of single factor test.The results showed that the flavone of Pueraria had strong scavengingeffect on H2O2 radical(IC50=1.8 μg/mL),and the optimal conditions by orthogonal matrix were in the following:Luminol: 0.05 mmol/L,H2O2: 0.01mol/L,pH 9.8.