Study on Extracting Total Flavone from PuerariaLobata and Its Property of Antioxidation

[Objective] The aim was to optimize the best technical conditions of extracting total flavone from Pueraria lobata,and studied its antioxidation property.[Method] The total flavone from P.lobata was extracted with ethanol,the factors that influenced the extraction rate of total flavone from P.lobata was studied through orthogonal experiment,and the optimal extracting process parameters were identified.The extract of total flavone from P.lobata was purified by using silica gel column chromatography,and the antioxidation property of P.lobata total flavone was determined with BPCL method.[Result] The orthogonal experiment showed that the sequence of four factors that affected the extraction rate of total flavone from P.lobata was:temperature ethanol concentration solid-liquid ratio extracting times.The optimal technical conditions of extracting total flavone from P.lobata were:ethanol concn.of 70%,solid-liquid ratio of 1∶8,extracting at 80 ℃ for times with 3 h per time,under which,the extraction rate of total flavone from P.lobata was 22.9%,with the sample purity of 15.5%.[Conclusion] The BPCL method was utilized to determine the inhibitory rate of total flavone from P.lobata to superoxide free radical(O2-·),and hydroxyl radical(·OH),and the result demonstrated that the total flavone from P.lobata had a certain antioxidant effects.