Expression of IFN-γ in peripheral blood mononuclear cells and spleen of aplastic anemia mice

Objective To explore the expression of IFN-γ in peripheral blood mononuclear cells(PBMC) and spleen of aplastic anemia mice,find the role of IFN-γ in immune pathogenesis of immune-mediated aplastic anemia mice.Methods ①Immunologically mediated method was used to imitate the model of aplastic anemia in BALB/C mice.②The mRNA expression of IFN-γ in PBMC of the model of aplastic anemia in BALB/C mice was measured by RT-PCR.③Protein expressions of IFN-γ in spleen of aplastic anemia mice model were detected by immune histochemistry.Results ①The mRNA expression of IFN-γ in peripheral blood mononuclear cells in aplastic anemia group was higher than that in the normal control group(1.14±0.06 vs 0.73±0.11,P0.05).②The rate of protein expression of IFN-γ in spleen of aplastic anemia group was(4.55±0.60)%,the normal group was(0.82±0.25)%,aplastic anemia group was significantly higher than normal control group(P0.01).Conclusion IFN-γ participate in the immune pathogenesis of aplastic anemia model.