2009•Shanxi Yike Daxue xuebaoRequires access
Expression of Foxp3 in aplastic anemia mice and its significance
Liu Chen
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Abstract
Objective To explore the expression of transcription factor Foxp3 of regulatory T-cell in peripheral blood mononuclear cells in aplastic anemia mice.Methods ①Immune-mediated method(γ-ray radiation and lymphocytes injection)was used to establish the model of aplastic anemia in BALB/c mice.②The expression of Foxp3 mRNA in peripheral blood mononuclear cells in aplastic anemia mice was measured by RT-PCR.③The expression of Foxp3 protein in spleen of aplastic anemia mice was detected by immunohistochemical method.Results ①The expression of Foxp3 mRNA in peripheral blood mononuclear cells in aplastic anemia mice was lower than that in normal mice(18.90±2.45 vs 57.27±5.71,P0.05).②The expression of Foxp3 protein in the spleen of aplastic anemia mice was lower than that in normal mice(19.2±10.0 vs 51.4±38.8,P0.05).Conclusion The results suggest that the expression of Foxp3 may be involved in the immune pathogenesis of aplastic anemia.