Screening of Different Carbon and Nitrogen Sources and Suitable for Fermentation on Five Strains of Lyophyllum decastes

Taking Lyophyllum decastes as material,the effects of different C and N-sources on mycelial biomass and exopolysaccharide production by five strains of Lyophyllum decastes were studied by single factor liquid fermentation experiment.The aim was to screen the optimum C-sources,N-sources and suitable fermenting strains for Lyophyllum decastes liquid culture,which was the basis of exploring its main factory fermentation.The results showed that the best C-source of strains ZY01,ZY02and ZY03was sucrose,the best N-source was peptone,followed by offals,urea.The mycelium biomass of strains ZY04and ZY05was most with glucose and peptone as optimum C and N-sources,sucrose was the best C-sources for its exopolysaccharide yield,the best N-sources was respectively urea,peptone.The mycelium biomass(12.626g/L)and exopolysaccharide production(11.5148mg/L)of strain ZY02was most with combination of 2%sucrose+0.2% peptone and 2%sucrose+0.3%peptone,respectively.Therefore,the effect of different C and Nsources on mycelial biomass and exopolysaccharide production was not a positive correlation,strain ZY02and ZY03was respectively the suitable fermenting strains of two kinds of indicators.