Studies on the Production of Mycelium by Lyophyllum decastes in Submerged Culture

Cultural and nutritional conditions for Lyophyllum decastes and its chemical composition in a synthetic medium were investigated. The optimal temperature and pH for the production of mycelium were and pH 7.5. The mycelium yield was the highest on 15th day. Among the carbon sources, glucose and CMC were the best for the production of mycelium and their optimal concentrations were 3 and 6%, respectively. As an organic nitrogen, proteose peptone was the best and as an inorganic nitrogen was good. The optimal concentration of proteose peptone and were 2 and 0.2%, respectively. The optimal ratio of glucose to proteose peptone for production of mycelium was 10 : 1. Also, the optimal concentrations of and were 0.2 and 0.06%, respectively and that of was 0.1%. Among the bioextracts, yeast extract was the most effective and its optimal concentration was 1.5%. In chemical components of the mycelium of Lyophyllum decastes, total sugar, crude protein and crude fat were 34.80, 28.35 and 2.50%, respectively. Its ash was 7.57% and crude fiber 11.99%.