Optimization of fermentation conditions and medium components for pilot production of Lyophyllum decastes

Based on the previous results of shake-flask fermentation conditions of Lyophyllum decastes mycelia,fermentation conditions and medium components of L.decastes Mycelia were optimized by uniform design with 200L fermenter.The results showed that optimum conditions of pilot fermentation were as follows: agitation speed 160r/min;fermenter pressure 0.2MPa;ventilation 80L/h;pH value 6.5;temperature 20℃;and inoculum size 10%.The optimum medium components were as follows: corn flour 5%;soybean 0.5%,ZnSO4 0.025%;MgSO4 0.05%;and KH2PO4 0.05%.Yield of mycelia was maximum on the eighth day(10.578g/L),and the content of exopolysaccharides was maximum on the ninth day(1.212g/L).