Inhibition of curcumin on proliferation of human osteosarcoma U-2OS cells

Objective To observe the inhibition of different concentrations of curcumin on cell growth,and explore the molecular mechanism of curcumin on human osteosarcoma.Methods Curcumin of different concentrations(0,10,20 and 40μmol/L) was administered to U-2OS cells of human osteosarcoma for 12h,24h and 48h,respectively.The proliferation of U-2OS cells was measured by MTT assay.Results Curcumin significantly inhibited the proliferation of PC-3 cells in vitro in concentration-and time-dependent manners.The higher the concentration of curcumin,the stronger the inhibition was(10μmol/L,20μmol/L,40μmol/L of r=-0.9638,-0.9778 and-0.9764,respectively,P0.05).With the prolonged administration,curcumin's inhibition on the U-2OS cells became more obvious(Linear correlation analysis showed 12h,24h and 48h of r=-0.9325,-0.9780 and-0.9705,respectively,P0.05).Conclusion Curcumin can significantly inhibit the proliferation of U-2OS 20S cells in vitro.Curcumin could provide a novel method in the clinical therapy for human osteosarcoma.