The Effect of Xuesetong on Neuronal Apoptosis and Expression of Bax, Bc1-2 in Cerebral Cortex Following Ischemi

In order to explore the change of number on neuronal apoptosis and expression of Bax, Bc1-2 in cerebral cortex following ischemia, the rats were performed local ischemia operation derived from middle cerebral artery block. Rats both Xuesetong treatment group and ischemia group were sacrificed and the cerebral cortex was taken. The tissues were sectioned into 20μm frozen sections. The sections were stained by DNA in situ terminal labeling technique (TUNEL) and ABC immunohistochemiscal method by antibody of Bax and Bc1-2. The number of apoptosis neurons and average gray degree for Bax, Bc1-2 was counted in each group. Results: The number of apoptosis neurons in Xuesetong group decreased greatly than that of ischemia group,, while the gray degree for Bax and Bc1-2 in Xuesetong group increased and decreased than that of ischemia group respectively. It showed that Bax expression was downregulated, but expression of Bc1-2 was upregulated following Xuesetong administration. Conclusion: Treatment of Xuesetong exerts protective effects on cerebral cortex neuron following ischemia.