Change of Number on Neuronal Apoptosis in Cerebral Cortex in Different Time Following Local Ischemi

In order to explore the change of number on neuronal apoptosis in cerebral cortex following ischemia, the rats were performed local ischemia operation derive from middle cerebral artery block. Rats were sacrificed at 1?h, 3?h, 6?h, 12?h following ischemia. The cerebral cortex was taken and sectioned into 20?μm frozen section. The sections were stained in situ terminal labeling technique (TUNEL). The number of apoptosis neurons was counted in each group. Results: The number of apoptosis neurons in control and 1?h, 3?h, 6?h, 12?h following ischemia is respectively 7±2, 37±7,49±5,63±7,89±7. It showed that apoptosis was trigged by ischemia in cerebral cortex. The number of apoptosis gradually increases related to the ischemia time.