Optimization of the ultrasonically assisted production of lutein by response surface method

Lutein was achieved using lutein esters by saponification technology were systemically investigated under ultrasonically conditions.Based on the single factor experiments,the affecting factors such as the water/solid ratio ( mL/g) ,reaction temperature and reaction time were optimized with response surface method ( RSM) . The impacts of factors on the yield of lutein were analyzed.The results showed that the optimal saponification parameter was the reaction at the water/solid ratio of 15( mL/g) ,48℃ and 138min.Under the optimum conditions,the yield of lutein can reach 75.8mg·g -1.