Technical Study of the Microwave Extraction of the Total Flavonoids from Hippophae Rhamnoides

Objective To study the optimal extraction condition of the flavones from Hippophae Rhamnoide with microwave method.Methods The petroleum ether was used for the derosination of Hippophae Rhamnoides at first.Afterward,on the basis of the single-factor test,by taking ethanol concentration,microwave power,extraction time and feed liquid ratio as the factors,at different levels,taking the extraction rate of the total flavonoids from Hippophae Rhamnoides as the assessment indicator,the orthogonal test was applied to optimize the extraction techniques.Results Of the four factors optioned in the trial,the ethanol concentration,and feed liquid ratio presented the significant impacts on the extraction rate of the total flavonoids from Hippophae Rhamnoides.The impacts of microwave power and extraction time on the trial results were not significant.The optimal extraction technical conditions of the total flavonoids from Hippophae Rhamnoides were 75% of ethanol concentration,300 w in microwave power,2 min in the extraction time and 1∶ 20 in feed liquid ratio.The extraction rate of the total flavonoids from Hippophae Rhamnoides could be up to 3.9%.Conclusion The microwave extraction method is advantageous as short extraction time,high extraction efficiency and less destruction of the active ingredients of Chinese medicine in the extraction of the total flavonoids from Hippophae Rhamnoides.