Transformation of-Bt(cryIA)-Gene into Soybean via Agrobacterium-mediated Method

It is very important for diseases and insect pests controlling to transfer some resistant genes to obtain transgenic resistant varieties.In this study,plant expression vector pCAMBIA3300-Bt containing-Bt(cryⅠA)-gene was constructed,and transgenic plants were obtained by Agrobacterium-mediated transformation of soybean(Heinong 37) cotyledonary nodes.We regenerated plants and optimized the conditions of transformation to increase the efficiency of transformation.It had the optimal rate of shooting when the concentration of 6-BA was 1.7 mg·L-1.The optimal selection concentration of Glufosinate-ammonium for shoot initiation was 3.5 mg·L-1.Transformed plants containing pCAMBIA3300-Bt was obtained and 19 positive plants from T1generation were tested by PCR.Real-time PCR was used to investigate the transcription level of Bt gene in T1 transgenic plants,it confirmed the integration of Bt genes into the genome of soybean.