Experimental study on protective effect of sTNFR genes in the hepatic ischemia reperfusion of rat

Objective To investigate the process and mechanisms in the hepatic ischemia/reperfusion injury of rat,and the protective effect of sTNFR genes.Methods Constitute the model of ischemia/reperfusion injury in the rat,then the rats were subjected to 60 min of sustained occlusion of the blood for left and middle liver followed by 1 hours,3 hours,6 hours and 12 hours of reperfusion.Detect the activity of alanine ALT and AST in the blood;the activity of malondialdehyde(MDA)in the liver tissues were assessed.The microscopical structure of the liver tissue were observed by HE staining.Results The activities of ALT,AST were all obviously damaged at the end of reperfusion respectively in the I/R group of rat.At the point of reperfusion 6 h,hepatic injury was the most serious;The activity of MDA in I/R group was higher than Sham group (P0.01),and reach the lowest point at after reperfusion 6 h.The expression of TNF-α was lower than the same time of I/R group(P0.05).Conclusions These data indicate that adenovirus-mediated sTNFR gene has protective effect against hepatic ischemia/reperfusion injury by enhancing the ability of eliminating oxygen free radicals;inhibiting the activation and expression of inflammation.