Content Determiniation of Protopine in Corydalis Rhizoma from Different Origions

Objective: To establish a new method for determining the content of protopine in Corydalis Rhizome,and set up the limitation standard of protopine through determining the content of the samples from different origions.Method: HPLC method was used.The separation was performed on a Aglient TC-C18 column(4.6 mm × 250 mm,5 μm) with mobile phase of acetonitrile-3% glacial acetic acid solution(contain 0.8% triethylamine)(18 ∶ 82).The flow rate was 1.0 mL.min-1and the detection wavelength was set at 289 nm.Result: The calibration curves showed good linear regression within test ranges of 0.052-0.52 μg;the recovery was 99.44%.Conclusion: The method established for determining the content of protopine in Corydalis rhizome was accurate,simple and feasible.The content of protopine of the samples from different origions varied from 0.065% to 0.199% with the average content of 0.124%.It is suggested that the suitable content standard of Corydalis rhizoma should be no less than 0.09%.