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Determination of ginsenoside Rg_1 in Nengennuoqi capsule by HPLC

Zhang Wen-jie

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Abstract

OBJECTIVE To establish an HPLC method for the determination of ginsenoside Rg 1 in Nengennuoqi capsule.METHODS An HPLC method was used with the following conditions: C 18 column (150 mm×4.6 mm, 5 μm), mobile phase was: acetonitrile - 0.1%phosphoric acid (19.4:80.6), detective wavelength was 203 nm.RESULTS A good linear relationship was found between concentration and peak area. The average recovery was 99.55% ( n =6).CONCLUSION This method showed a good resolution and reliability.

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OBJECTIVE To establish an HPLC method for the determination of ginsenoside Rg 1 in Nengennuoqi capsule.METHODS An HPLC method was used with the following conditions: C 18 column (150 mm×4.6 mm, 5 μm), mobile phase was: acetonitrile - 0.1%phosphoric acid (19.4:80.6), detective wavelength was 203 nm.RESULTS A good linear relationship was found between concentration and peak area. The average recovery was 99.55% ( n =6).CONCLUSION This method showed a good resolution and reliability.

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Available abstract

OBJECTIVE To establish an HPLC method for the determination of ginsenoside Rg 1 in Nengennuoqi capsule.METHODS An HPLC method was used with the following conditions: C 18 column (150 mm×4.6 mm, 5 μm), mobile phase was: acetonitrile - 0.1%phosphoric acid (19.4:80.6), detective wavelength was 203 nm.RESULTS A good linear relationship was found between concentration and peak area. The average recovery was 99.55% ( n =6).CONCLUSION This method showed a good resolution and reliability.

Key concepts: Chemistry, Capsule, High-performance liquid chromatography, Phosphoric acid, Chromatography, Resolution (logic), Acetonitrile, Ginsenoside Rg1

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