Effect of Bushen and Huoxue Method on MMP-13 and ADAMTS-5 in Synovial Membrane and Cartilage of SD Rats with Knee Osteoarthritis

Objective:To detect the effects of Bushenhuoxue,Huoxue,Bushen,and Qufeng method on the expression of IL-1β,TNF-α,MMP-13,and ADAMTS-5 in SD rats with knee osteoarthritis.Methods: All 180 SD rats were randomly divided into 6 groups: Huoxue group,Qufeng group,Bushen group,Bushenhuoxue group,preventive group,and model group,with 30 rats in each group.The model of knee osteoarthritis was made by injecting 4% papain in the knee joint.The rats were continuous administration after modeling successfully,one month for a treatment course.Ten rats in each group were killed and the synovium and knee cartilage were taken in 30,60,and 90 days respectively after treatment.The levels of IL-1β and TNF-α in synovium were detected by immunohistochemistry,while,the expression levels of MMP-13 and ADAMTS-5 mRNA in cartilage were detected by real-time quantitative PCR.Results:The levels of IL-1β,TNF-α and MMP-13,ADAMTS-5 mRNA in the model group and Qufeng group were significantly higher than the other groups,but the lower was not obvious as the treatment progresses.In the early stage,the levels were lowest in the preventive group(P0.05).There was no difference between Huoxue group and BushenHuoxue group,but they were lower than the other groups except preventive group(P0.05).In the later stage,the levels in Bushenhuoxue group and Bushen group were decreased significantly than other groups(P0.05),and there was significant difference between Bushenhuoxue group and Bushen group(P0.05).Conclusion:Bushen and Huoxue method plays different role in the expression of IL-1β,TNF-α and MMP-13,ADAMTS-5 mRNA in rats with knee osteoarthritis at different stage.In the early stage,the Huoxue method plays an important role.In the later stage,the Bushen method is more effective than the Huoxue method,and the Bushenhuoxue method is the most effective treatment.