Influence of Bushen Huoxue Duiyao on the Level of MMP-3 and TIMP-1 of Rabbits with Knee Osteoarthritis

Objective To investigate the mechanism of reinforcing kidney and promoting blood circulation couple herbs regulating rabbit cartilage and synovial membrane of matrix metalloproteinases(MMPS-3) and its inhibitor(TIMP-1) in knee osteoarthritis model,to screen out the osteoarthritis target specific treatments.Method Forty-eight healthy Japanese white rabbit were randomly divided into control group,model group,Bushen duiyao group,Huoxue duiyao group,Bushen Huoxue duiyao group,and Bushen Huoxue medicine group,8 rabbits in each group.Model of knee osteoarthritis was made by 1.6% papain saline solution intra-articular injection.Continuous administration of 42 days later,expression of MMPS-3 and TIMP-1 mRNA of rabbit knee joint cartilage and synovial membrane was measured by RT-PCR method.Result Cartilage MMP-3 of the model group was significantly higher than the other five groups(P0.01),Huoxue duiyao group was significantly higher than the control group(P0.01);TIMP-1 of the control group was significantly lower than Bushen Huoxue duiyao group(P0.05),Bushen duiyao group and Bushen Huoxue medicine group were significantly higher than model group(P0.01).Synovial MMP-3 of the model group was significantly higher than the other groups(P0.01),Bushen duiyao and Huoxue duiyao group had no significant difference(P0.05);TIMP-1 of control group was significantly lower than model group and treatment groups(P0.01),Bushen huoxue medicine group was significantly higher than model group(P0.01),Bushen Huoxue duiyao group was significantly higher than the model group(P0.01).Conclusion Bushen Huoxue medicine group can decrease MMP-3 expression in serum and increase TIMP-1 level slightly.This hints that Bushen Huoxue duiyao and Bushen Huoxue medicine group can maintain the MMP-3 and TIMP-1 gene expression ratio,while Bushen Huoxue medicine group is more effective than Bushen Huoxue duiyao group.