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Assaying Total Flavonoids in Black Corn Pollen by Double-wavelength Spectrophotometry

Weihua Zhang

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Abstract

In this paper, a double-wavelength spectrophotometry was applied to assay the total flavonoids in black corn pollen. The detective wavelength was 510 nm ,while the reference one was 584 nm, and the recovery rate was 98.14%, with RSD=1.24% (n=5).The results indicated that the average total flavonoids content in black corn pollen was 3.53%, and the method was stable, reliable and accurate in the assay process.

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What this paper is about

In this paper, a double-wavelength spectrophotometry was applied to assay the total flavonoids in black corn pollen. The detective wavelength was 510 nm ,while the reference one was 584 nm, and the recovery rate was 98.14%, with RSD=1.24% (n=5).The results indicated that the average total flavonoids content in black corn pollen was 3.53%, and the method was stable, reliable and accurate in the assay process.

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Available abstract

In this paper, a double-wavelength spectrophotometry was applied to assay the total flavonoids in black corn pollen. The detective wavelength was 510 nm ,while the reference one was 584 nm, and the recovery rate was 98.14%, with RSD=1.24% (n=5).The results indicated that the average total flavonoids content in black corn pollen was 3.53%, and the method was stable, reliable and accurate in the assay process.

Key concepts: Spectrophotometry, Pollen, Chemistry, Chromatography, Food science, Botany, Biology

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