Development and characterization of mouse monoclonal antibodies reactive with chicken TL1A (VET2P.1029)

Abstract Two mouse monoclonal antibodies (mAbs) which specifically detect chicken TL1A (chTL1A) were developed and characterized. Both mAbs identified a 32 kDa E. coli-derived, poly-histidine-tagged fusion protein by Western blot analysis. Both mAbs identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry, and were used to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens by antigen capture ELISA. These mAbs inhibited chTL1A-induced spleen lymphocyte proliferation, nitric oxide production by HD11 chicken macrophages, and blocked the cytotoxic effect of chTL1A against LSCC-RP9 chicken B tumor cells. These new mAbs detecting chTL1A will be important immune reagents for basic and applied research in poultry immunology