Determination of chlorogenic acid and rutin in Ainsliaea fragrans by RP-HPLC with gradient elution

A method for simultaneous determination of chlorogenic acid and rutin in Ainsliaea fragrans from different resources was established.A kromasil C18(4.6 mm×250 mm,5 μm) column was used with a mobile phase consisted of phase A (methanol)-phase B(0.5% phosphoric acid water solution) in gradient elution 0 min(30∶70)-15 min(30∶70)-20 min(50∶50)-40 min(50∶50) at a flow rate of 0.8 mL/min in this method,the detection wavelength was set at 350 nm and the column temperature was 25 ℃.Photodiode array detector was used.The calibration curve was linear in a range of 0.654～5.886 μg for chlorogenic acid(r=0.9997).The average recovery was 98.6% and RSD was 1.7%(n=6).The calibration curve was linear in a range of 0.109～0.981 μg for rutin(r=0.9993).The average recovery was 97.1% and RSD was 2.3%(n=6).This method is rapid,simple and reproducible.It can be used as a quality control method for rutin and chlorogenic acid in Ainsliaea fragrans.