Optimizing for Vigorous Seedling Culture System for the Virus-free Tube Potato Plantlets

Effects of the various combinations of NAA,B9,6-BA and macro-element on the potato plantlets in vitro of cv.Favorita were studied by using the orthogonal experiment design in this research,which would provide a theoretical reference for the establishment of optimized culture system for strengthening plantlets in vitro.Effect order of the factors on stem diameter,root number,root length and plant dry weight was macro-elementB9NAA6-BA.For plantlet height,node number,leaf number and fresh weight,macro-element was most influencing,while NAA least influencing.For node number,leaf number,stem diameter,root length,root number,and fresh anddry weight per plantlet in vitro,all treatments but four were highly significantly different from the control.The medium,MS + NAA 0.03 mg/L + B9 10 mg/L + white sugar 2% + agar 0.6%,except root length,gave the highest value,suggesting that this medium is most suitable for 'Favorita' to produce robust plantlets in vitro.